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KMID : 0381120230450040507
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Genes and Genomics
2023 Volume.45 No. 4 p.507 ~ p.517
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Knockdown of growth differentiation factor-15 inhibited nonsmall cell lung cancer through inactivating PTEN/PI3K/AKT signaling pathway
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Yongshi Liu
Jie Lei
Xiang Ji
Chunmei Li
Xiaoxia Chen
Juan Wang
Jiajia Dong
Hongpei Zhang
Yan Li
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Abstract
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Background : Non-small cell lung cancer (NSCLC) is characterized by high morbidity and mortality in the world. Growth and differentiation factor 15 (GDF15) has been proved to play an important role in regulating tumor progression. However, the influence of GDF15 on NSCLC remains unclear.
Objective : We aimed to investigate the regulatory role of GDF15 in NSCLC.
Methods : The correlation between GDF15 expression and prognosis, stage of NSCLC was examined with bioinformatics method. The cell proliferation was detected with CCK8 and EdU staining. Wound healing, Transwell, flow cytometry assays were used to measure cell migration, invasion, and apoptosis, respectively.
Results : High expression of GDF15 is correlated with worse survival and malignant progression of NSCLC. Knockdown of GDF15 restrained the proliferation, invasion, migration, but accelerated apoptosis of lung cancer cells through regulating PTEN/PI3K/AKT signaling pathway. sh-GDF15 suppressed epithelial mesenchymal transition (EMT) process and promoted the chemotherapy sensitivity of lung cancer cells.
Conclusion : GDF15 plays an important role in NSCLC progression. GDF15 mediated PTEN/PI3K/AKT signaling pathway might be the potential therapeutic targets for the prevention and treatment of GDF15.
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KEYWORD
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GDF15, NSCLC, PTEN/PI3K/AKT, EMT
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